Summary: During sleep, breathing entrains and coordinates neural activity across the limbic system, and enhances memory consolidation.
While we sleep, the brain is not switched off, but is busy with “saving” the important memories of the day. To achieve that, brain regions are synchronized to coordinate the transmission of information between them. Yet, the mechanisms that enable this synchronization across multiple remote brain regions are not well understood.
Traditionally, these mechanisms were sought in correlated activity patterns within the brain. However, LMU neuroscientists Prof. Anton Sirota and Dr. Nikolas Karalis have now been able to show that breathing acts as a pacemaker that entrains the various brain regions and synchronizes them with each other.
Breathing is the most persistent and essential bodily rhythm and exerts a strong physiological effect on the autonomous nervous system. It is also known to modulate a wide range of cognitive functions such as perception, attention, and thought structure. However, the mechanisms of its impact on cognitive function and the brain are largely unknown.
The scientists performed large-scale in vivo electrophysiological recordings in mice, from thousands of neurons across the limbic system. They showed that respiration entrains and coordinates neuronal activity in all investigated brain regions – including the hippocampus, medial prefrontal and visual cortex, thalamus, amygdala, and nucleus accumbens – by modulating the excitability of these circuits in olfaction-independent way.
“Thus, we were able to prove the existence of a novel non-olfactory, intracerebral, mechanismthat accounts for the entrainment of distributed circuits by breathing, which we termed “respiratory corollary discharge”, says Karalis, who is currently research fellow at the Friedrich Miescher Institute for Biomedical Research in Basel.
“Our findings identify the existence of a previously unknown link between respiratory and limbic circuits and are a departure from the standard belief that breathing modulates brain activity via the nose-olfactory route”, underlines Sirota.
This mechanism mediates the coordination of sleep-related activity in these brain regions, which is essential for memory consolidation and provides the means for the co-modulation of the cortico-hippocampal circuits synchronous dynamics. According to the authors, these results represent a major step forward and provide the foundation for new mechanistic theories, that incorporate the respiratory rhythm as a fundamental mechanism underlying the communication of distributed systems during memory consolidation.
Breathing coordinates cortico-hippocampal dynamics in mice during offline states
Network dynamics have been proposed as a mechanistic substrate for the information transfer across cortical and hippocampal circuits. However, little is known about the mechanisms that synchronize and coordinate these processes across widespread brain regions during offline states.
Here we address the hypothesis that breathing acts as an oscillatory pacemaker, persistently coupling distributed brain circuit dynamics. Using large-scale recordings from a number of cortical and subcortical brain regions in behaving mice, we uncover the presence of an intracerebral respiratory corollary discharge, that modulates neural activity across these circuits.
During offline states, the respiratory modulation underlies the coupling of hippocampal sharp-wave ripples and cortical DOWN/UP state transitions, which mediates systems memory consolidation.
These results highlight breathing, a perennial brain rhythm, as an oscillatory scaffold for the functional coordination of the limbic circuit that supports the segregation and integration of information flow across neuronal networks during offline states.